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anti tropomyosin receptor kinase b trkb  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti tropomyosin receptor kinase b trkb
    Anti Tropomyosin Receptor Kinase B Trkb, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+trka+antibody/Phospho-TrkA+(Tyr674%2F675)%2FTrkB+(Tyr706%2F707)+Rabbit+mAb/pm42884630-72-47-53
    Average 95 stars, based on 118 article reviews
    anti tropomyosin receptor kinase b trkb - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Immunoprecipitation:

    Article Title: Sustained GRK2-dependent CREB activation is essential for α 2 -adrenergic receptor-induced PC12 neuronal differentiation.
    Article Snippet: Many aspects of neuronal development, such as neuronal survival and differentiation, are regulated by the transcription factor cAMP-response element-binding protein (CREB).. We have previously reported that 2-adrenergic receptors (ARs), members of the G protein-coupled receptor (GPCR) superfamily, induce neuronal differentiation of rat pheochromocytoma (PC)-12 cells in a subtype-specific manner, i.e. 2A< 2B< 2C.. Herein, we sought to investigate CREB`s involvement in this Jo ur na l P re -p oo f

    Western Blot:

    Article Title: Sustained GRK2-dependent CREB activation is essential for α 2 -adrenergic receptor-induced PC12 neuronal differentiation.
    Article Snippet: Many aspects of neuronal development, such as neuronal survival and differentiation, are regulated by the transcription factor cAMP-response element-binding protein (CREB).. We have previously reported that 2-adrenergic receptors (ARs), members of the G protein-coupled receptor (GPCR) superfamily, induce neuronal differentiation of rat pheochromocytoma (PC)-12 cells in a subtype-specific manner, i.e. 2A< 2B< 2C.. Herein, we sought to investigate CREB`s involvement in this Jo ur na l P re -p oo f

    other:

    Article Title: Neuroblast differentiation during development and in neuroblastoma requires KIF1Bβ-mediated transport of TRKA
    Article Snippet: Biotinylated proteins were resolved by SDS-PAGE, transferred to a nitrocellulose filter, and immunoblotted with an anti-TrkA antibody (1:1000; Cell Signaling, catalog no. 2505), anti-EGFR antibody (1:1000; Cell Signaling, catalog no. 2232), or anti-TNFR1 antibody (1:1000; Cell Signaling, catalog no. 3736).

    Membrane:

    Article Title: NGF-conjugated iron oxide nanoparticles promote differentiation and outgrowth of PC12 cells.
    Article Snippet: The search for regenerative agents that promote neuronal differentiation and repair is of great importance.. Nerve growth factor (NGF) which is an essential contributor to neuronal differentiation has shown high pharmacological potential for the treatment of central neurodegenerative diseases such as Alzheimer’s and Parkinson’s.. However, growth factors undergo rapid degradation, leading to a short biological half-life.

    Article Title: Precautionary effects of Red Liriope platyphylla on NGF secretion and Aβ42 deposition under the preclinical stage of Alzheimer's disease in Tg2576 mice
    Article Snippet: The reaction was then quenched by the addition of 100 μL of stop solution, after which the plates were analyzed by a SoftMax Pro5 spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). .. Proteins prepared from the brain tissues of Non-Tg, vehicle- and AEtRLP-treated Tg2576 mice were separated by electrophoresis on a 10-20% SDS-PAGE gel for 2 h, then transferred to nitrocellulose membranes for 2 h at 40 V. Next, each membrane was incubated separately with one of the following primary antibodies: anti-TrkA antibody (Cell Signaling Technology, Beverley, MA, USA), anti-p-TrkA antibody (Cell Signaling Technology), anti-Akt antibody (Cell Signaling Technology), anti-p-Akt antibody (Cell Signaling Technology), anti-ERK antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-p-ERK antibody (Santa Cruz Biotechnology), anti-p75 NTR antibody (Cell Signaling Technology), anti-RhoA antibody (Cell Signaling Technology), anti-Bax antibody (Abcam, Cambridge, UK), anti-Bcl-2 (Abcam), or anti-beta actin (Sigma-Aldrich, St. Louis, MO, USA). .. Each membrane was then washed with buffer (137 mM NaCl, 2.7 mM KCl, 10 mM NaHPO 4 , and 0.05% Tween-20) and incubated with a 1:1,000 dilution of horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG at room temperature for 2 h. Finally, the membrane blots were developed using an Enhanced Chemiluminescence (ECL) Reagent Plus kit (Amersham Life Science, Piscatway, NJ, USA).

    Article Title: Asparagus cochinchinensis stimulates release of nerve growth factor and abrogates oxidative stress in the Tg2576 model for Alzheimer's disease.
    Article Snippet: Western blot and slot blot Total protein was prepared from the cortex and hippocampus tissue of mice in subset groups using Pro-Prep Protein Extraction Solution (iNtRON Biotechnology, Seongnam, Korea), then quantified using a SMARTTM BCA Protein Assay Kit (Thermo Scientific, Waltham, MA, USA) for Western blot analysis. .. Briefly, these proteins were separated by 4–20% sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) for 2 h, after which resolved proteins were transferred to nitrocellulose membranes for 2 h at 40 V. Each membrane was then incubated separately at 4 °C with the following primary antibodies overnight: anti-TrkA antibody (Cell Signaling Technology, Beverley, MA, USA), antip-TrkA antibody (Cell Signaling Technology), anti-Akt antibody (Cell Signaling Technology), anti-p-Akt antibody (Cell Signaling Technology), anti-ERK antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-p-ERK antibody (Santa Cruz Biotechnology), antip75NTR antibody (Cell Signaling Technology), anti-JNK antibody (Cell Signaling Technology), anti-p-JNK antibody (Cell Signaling Technology) and anti-actin antibody (Sigma-Aldrich Co.). .. Next, the membranes were washed with washing buffer (137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, and 0.05% Tween 20), then incubated with 1:1000 diluted horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (Invitrogen, Carlsbad, CA, USA) at room temperature for 1 h. Finally, membrane blots were developed using Amersham ECL Select Western Blotting detection reagent (GE Healthcare, Little Chalfont, UK).

    Article Title: Asparagus cochinchinensis stimulates release of nerve growth factor and abrogates oxidative stress in the Tg2576 model for Alzheimer’s disease
    Article Snippet: Total protein was prepared from the cortex and hippocampus tissue of mice in subset groups using Pro-Prep Protein Extraction Solution (iNtRON Biotechnology, Seongnam, Korea), then quantified using a SMARTTM BCA Protein Assay Kit (Thermo Scientific, Waltham, MA, USA) for Western blot analysis. .. Briefly, these proteins were separated by 4–20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for 2 h, after which resolved proteins were transferred to nitrocellulose membranes for 2 h at 40 V. Each membrane was then incubated separately at 4 °C with the following primary antibodies overnight: anti-TrkA antibody (Cell Signaling Technology, Beverley, MA, USA), anti-p-TrkA antibody (Cell Signaling Technology), anti-Akt antibody (Cell Signaling Technology), anti-p-Akt antibody (Cell Signaling Technology), anti-ERK antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-p-ERK antibody (Santa Cruz Biotechnology), anti-p75 NTR antibody (Cell Signaling Technology), anti-JNK antibody (Cell Signaling Technology), anti-p-JNK antibody (Cell Signaling Technology) and anti-actin antibody (Sigma-Aldrich Co.). .. Next, the membranes were washed with washing buffer (137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , and 0.05% Tween 20), then incubated with 1:1000 diluted horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (Invitrogen, Carlsbad, CA, USA) at room temperature for 1 h. Finally, membrane blots were developed using Amersham ECL Select Western Blotting detection reagent (GE Healthcare, Little Chalfont, UK).

    Incubation:

    Article Title: Evaluation of NTRK Fusions Detection Method in Esophageal Squamous Cell Carcinoma and Gastric Adenocarcinoma
    Article Snippet: .. After rinsing in PBS, the sections were incubated with ananti-pan-TRK antibody (# EPR17341 ; 1:50 dilution; Abcam, Cambridge, UK), anti-TRKA antibody (#2510; 12G8; 1:400 dilution; Cell Signaling Technology, Danvers, MA, USA), anti-TRKB antibody (#4607; 80G2; 1:2000 dilution; Cell signaling Technology), or anti-TRKC antibody (#3376; C44H5; 1:1000 dilution; Cell Signaling Technology) at 4 °C overnight. .. Incubation was performed by a peroxidase-labeled polymer conjugated to goat antirabbit immunoglobulins (ENvision + kit; Dako, Agilent, Santa Clara, CA, USA) as the secondary antibody for 30 min at room temperature, after the PBS wash. For visualization, diaminobenzidine staining was performed, followed by counterstaining with hematoxylin and eosin (H&E).

    Article Title: Precautionary effects of Red Liriope platyphylla on NGF secretion and Aβ42 deposition under the preclinical stage of Alzheimer's disease in Tg2576 mice
    Article Snippet: The reaction was then quenched by the addition of 100 μL of stop solution, after which the plates were analyzed by a SoftMax Pro5 spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). .. Proteins prepared from the brain tissues of Non-Tg, vehicle- and AEtRLP-treated Tg2576 mice were separated by electrophoresis on a 10-20% SDS-PAGE gel for 2 h, then transferred to nitrocellulose membranes for 2 h at 40 V. Next, each membrane was incubated separately with one of the following primary antibodies: anti-TrkA antibody (Cell Signaling Technology, Beverley, MA, USA), anti-p-TrkA antibody (Cell Signaling Technology), anti-Akt antibody (Cell Signaling Technology), anti-p-Akt antibody (Cell Signaling Technology), anti-ERK antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-p-ERK antibody (Santa Cruz Biotechnology), anti-p75 NTR antibody (Cell Signaling Technology), anti-RhoA antibody (Cell Signaling Technology), anti-Bax antibody (Abcam, Cambridge, UK), anti-Bcl-2 (Abcam), or anti-beta actin (Sigma-Aldrich, St. Louis, MO, USA). .. Each membrane was then washed with buffer (137 mM NaCl, 2.7 mM KCl, 10 mM NaHPO 4 , and 0.05% Tween-20) and incubated with a 1:1,000 dilution of horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG at room temperature for 2 h. Finally, the membrane blots were developed using an Enhanced Chemiluminescence (ECL) Reagent Plus kit (Amersham Life Science, Piscatway, NJ, USA).

    Article Title: Asparagus cochinchinensis stimulates release of nerve growth factor and abrogates oxidative stress in the Tg2576 model for Alzheimer's disease.
    Article Snippet: Western blot and slot blot Total protein was prepared from the cortex and hippocampus tissue of mice in subset groups using Pro-Prep Protein Extraction Solution (iNtRON Biotechnology, Seongnam, Korea), then quantified using a SMARTTM BCA Protein Assay Kit (Thermo Scientific, Waltham, MA, USA) for Western blot analysis. .. Briefly, these proteins were separated by 4–20% sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) for 2 h, after which resolved proteins were transferred to nitrocellulose membranes for 2 h at 40 V. Each membrane was then incubated separately at 4 °C with the following primary antibodies overnight: anti-TrkA antibody (Cell Signaling Technology, Beverley, MA, USA), antip-TrkA antibody (Cell Signaling Technology), anti-Akt antibody (Cell Signaling Technology), anti-p-Akt antibody (Cell Signaling Technology), anti-ERK antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-p-ERK antibody (Santa Cruz Biotechnology), antip75NTR antibody (Cell Signaling Technology), anti-JNK antibody (Cell Signaling Technology), anti-p-JNK antibody (Cell Signaling Technology) and anti-actin antibody (Sigma-Aldrich Co.). .. Next, the membranes were washed with washing buffer (137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, and 0.05% Tween 20), then incubated with 1:1000 diluted horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (Invitrogen, Carlsbad, CA, USA) at room temperature for 1 h. Finally, membrane blots were developed using Amersham ECL Select Western Blotting detection reagent (GE Healthcare, Little Chalfont, UK).

    Article Title: Asparagus cochinchinensis stimulates release of nerve growth factor and abrogates oxidative stress in the Tg2576 model for Alzheimer’s disease
    Article Snippet: Total protein was prepared from the cortex and hippocampus tissue of mice in subset groups using Pro-Prep Protein Extraction Solution (iNtRON Biotechnology, Seongnam, Korea), then quantified using a SMARTTM BCA Protein Assay Kit (Thermo Scientific, Waltham, MA, USA) for Western blot analysis. .. Briefly, these proteins were separated by 4–20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for 2 h, after which resolved proteins were transferred to nitrocellulose membranes for 2 h at 40 V. Each membrane was then incubated separately at 4 °C with the following primary antibodies overnight: anti-TrkA antibody (Cell Signaling Technology, Beverley, MA, USA), anti-p-TrkA antibody (Cell Signaling Technology), anti-Akt antibody (Cell Signaling Technology), anti-p-Akt antibody (Cell Signaling Technology), anti-ERK antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-p-ERK antibody (Santa Cruz Biotechnology), anti-p75 NTR antibody (Cell Signaling Technology), anti-JNK antibody (Cell Signaling Technology), anti-p-JNK antibody (Cell Signaling Technology) and anti-actin antibody (Sigma-Aldrich Co.). .. Next, the membranes were washed with washing buffer (137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , and 0.05% Tween 20), then incubated with 1:1000 diluted horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (Invitrogen, Carlsbad, CA, USA) at room temperature for 1 h. Finally, membrane blots were developed using Amersham ECL Select Western Blotting detection reagent (GE Healthcare, Little Chalfont, UK).

    Electrophoresis:

    Article Title: Precautionary effects of Red Liriope platyphylla on NGF secretion and Aβ42 deposition under the preclinical stage of Alzheimer's disease in Tg2576 mice
    Article Snippet: The reaction was then quenched by the addition of 100 μL of stop solution, after which the plates were analyzed by a SoftMax Pro5 spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). .. Proteins prepared from the brain tissues of Non-Tg, vehicle- and AEtRLP-treated Tg2576 mice were separated by electrophoresis on a 10-20% SDS-PAGE gel for 2 h, then transferred to nitrocellulose membranes for 2 h at 40 V. Next, each membrane was incubated separately with one of the following primary antibodies: anti-TrkA antibody (Cell Signaling Technology, Beverley, MA, USA), anti-p-TrkA antibody (Cell Signaling Technology), anti-Akt antibody (Cell Signaling Technology), anti-p-Akt antibody (Cell Signaling Technology), anti-ERK antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-p-ERK antibody (Santa Cruz Biotechnology), anti-p75 NTR antibody (Cell Signaling Technology), anti-RhoA antibody (Cell Signaling Technology), anti-Bax antibody (Abcam, Cambridge, UK), anti-Bcl-2 (Abcam), or anti-beta actin (Sigma-Aldrich, St. Louis, MO, USA). .. Each membrane was then washed with buffer (137 mM NaCl, 2.7 mM KCl, 10 mM NaHPO 4 , and 0.05% Tween-20) and incubated with a 1:1,000 dilution of horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG at room temperature for 2 h. Finally, the membrane blots were developed using an Enhanced Chemiluminescence (ECL) Reagent Plus kit (Amersham Life Science, Piscatway, NJ, USA).

    SDS Page:

    Article Title: Precautionary effects of Red Liriope platyphylla on NGF secretion and Aβ42 deposition under the preclinical stage of Alzheimer's disease in Tg2576 mice
    Article Snippet: The reaction was then quenched by the addition of 100 μL of stop solution, after which the plates were analyzed by a SoftMax Pro5 spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). .. Proteins prepared from the brain tissues of Non-Tg, vehicle- and AEtRLP-treated Tg2576 mice were separated by electrophoresis on a 10-20% SDS-PAGE gel for 2 h, then transferred to nitrocellulose membranes for 2 h at 40 V. Next, each membrane was incubated separately with one of the following primary antibodies: anti-TrkA antibody (Cell Signaling Technology, Beverley, MA, USA), anti-p-TrkA antibody (Cell Signaling Technology), anti-Akt antibody (Cell Signaling Technology), anti-p-Akt antibody (Cell Signaling Technology), anti-ERK antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-p-ERK antibody (Santa Cruz Biotechnology), anti-p75 NTR antibody (Cell Signaling Technology), anti-RhoA antibody (Cell Signaling Technology), anti-Bax antibody (Abcam, Cambridge, UK), anti-Bcl-2 (Abcam), or anti-beta actin (Sigma-Aldrich, St. Louis, MO, USA). .. Each membrane was then washed with buffer (137 mM NaCl, 2.7 mM KCl, 10 mM NaHPO 4 , and 0.05% Tween-20) and incubated with a 1:1,000 dilution of horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG at room temperature for 2 h. Finally, the membrane blots were developed using an Enhanced Chemiluminescence (ECL) Reagent Plus kit (Amersham Life Science, Piscatway, NJ, USA).

    Nucleic Acid Electrophoresis:

    Article Title: Asparagus cochinchinensis stimulates release of nerve growth factor and abrogates oxidative stress in the Tg2576 model for Alzheimer's disease.
    Article Snippet: Western blot and slot blot Total protein was prepared from the cortex and hippocampus tissue of mice in subset groups using Pro-Prep Protein Extraction Solution (iNtRON Biotechnology, Seongnam, Korea), then quantified using a SMARTTM BCA Protein Assay Kit (Thermo Scientific, Waltham, MA, USA) for Western blot analysis. .. Briefly, these proteins were separated by 4–20% sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) for 2 h, after which resolved proteins were transferred to nitrocellulose membranes for 2 h at 40 V. Each membrane was then incubated separately at 4 °C with the following primary antibodies overnight: anti-TrkA antibody (Cell Signaling Technology, Beverley, MA, USA), antip-TrkA antibody (Cell Signaling Technology), anti-Akt antibody (Cell Signaling Technology), anti-p-Akt antibody (Cell Signaling Technology), anti-ERK antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-p-ERK antibody (Santa Cruz Biotechnology), antip75NTR antibody (Cell Signaling Technology), anti-JNK antibody (Cell Signaling Technology), anti-p-JNK antibody (Cell Signaling Technology) and anti-actin antibody (Sigma-Aldrich Co.). .. Next, the membranes were washed with washing buffer (137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, and 0.05% Tween 20), then incubated with 1:1000 diluted horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (Invitrogen, Carlsbad, CA, USA) at room temperature for 1 h. Finally, membrane blots were developed using Amersham ECL Select Western Blotting detection reagent (GE Healthcare, Little Chalfont, UK).

    Article Title: Asparagus cochinchinensis stimulates release of nerve growth factor and abrogates oxidative stress in the Tg2576 model for Alzheimer’s disease
    Article Snippet: Total protein was prepared from the cortex and hippocampus tissue of mice in subset groups using Pro-Prep Protein Extraction Solution (iNtRON Biotechnology, Seongnam, Korea), then quantified using a SMARTTM BCA Protein Assay Kit (Thermo Scientific, Waltham, MA, USA) for Western blot analysis. .. Briefly, these proteins were separated by 4–20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for 2 h, after which resolved proteins were transferred to nitrocellulose membranes for 2 h at 40 V. Each membrane was then incubated separately at 4 °C with the following primary antibodies overnight: anti-TrkA antibody (Cell Signaling Technology, Beverley, MA, USA), anti-p-TrkA antibody (Cell Signaling Technology), anti-Akt antibody (Cell Signaling Technology), anti-p-Akt antibody (Cell Signaling Technology), anti-ERK antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-p-ERK antibody (Santa Cruz Biotechnology), anti-p75 NTR antibody (Cell Signaling Technology), anti-JNK antibody (Cell Signaling Technology), anti-p-JNK antibody (Cell Signaling Technology) and anti-actin antibody (Sigma-Aldrich Co.). .. Next, the membranes were washed with washing buffer (137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , and 0.05% Tween 20), then incubated with 1:1000 diluted horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (Invitrogen, Carlsbad, CA, USA) at room temperature for 1 h. Finally, membrane blots were developed using Amersham ECL Select Western Blotting detection reagent (GE Healthcare, Little Chalfont, UK).



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    Image Search Results


    Journal: Cell Reports Medicine

    Article Title: A biomimetic hybrid membrane vesicle nanoplatform attenuates tendinopathy through neuroinflammation modulation and tendon regeneration

    doi: 10.1016/j.xcrm.2026.102937

    Figure Lengend Snippet:

    Article Snippet: Rabbit Polyclonal anti-TrkA , ABclonal , Cat# A4147; RRID: AB_2863195.

    Techniques: Recombinant, Lysis, Hydroxyproline Assay, Enzyme-linked Immunosorbent Assay, Isolation, SYBR Green Assay, Bicinchoninic Acid Protein Assay, Staining, Software

    Journal: Stem Cell Reports

    Article Title: A highly efficient method to differentiate CGRP-expressing peptidergic nociceptors from human induced pluripotent stem cells

    doi: 10.1016/j.stemcr.2026.102971

    Figure Lengend Snippet:

    Article Snippet: Rabbit anti-TRKA , Alomone Labs , Cat# ANT-018; RRID: AB_10658910.

    Techniques: